| ISBN: | 9781071643853 |
| 语种代码: | eng |
| 个人名称: | Ortega, Felipe |
| 题名: | Neural stem cells : methods and protocols / edited by Felipe Ortega, Faculty of veterinary Medicine, Complutense Universiy of Madrid, Madrid, Madrid, Spain. |
| 出版发行项: | New York, NY : Humana Press, [2025] |
| 载体形态: | xvi, 391 pages : illustrations (chiefly color) ; 26 cm. |
| 书目附注: | Includes bibliographical references and index. |
| 格式化内容附注: | Flow-Cytometry-Based Protocols for the Mouse Subependymal Neurogenic Niche Phenotyping -- Isolation of Adult Hippocampal Neural Stem Cells -- Low Density Primary Cell Culture of Postnatal Murine Cerebellar Progenitors In Vitro -- Isolation of Oligodendrocyte Precursor Cells with Immunomagnetic Beads for Their Better Viability In Vitro and In Vivo -- Non-Hematopoietic Stem Cell Identification and Sorting in Adult Retina -- Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay -- Detection of Monocytic Myeloid-Derived Suppressor Cells in the Human Brain and Blood -- Cell-Type Specific Protein Metabolic Labeling and Identification Using the Methionine Subrogate ANL in Cells Expressing a Mutant Methionyl-tRNA Synthetase -- Experimental Neurogenesis in the Embryos of the Gecko Paroedura picta -- Genetic Tracing of Progenitors from Embryo to Postnatal Brain -- Tracking Neural Migration in Dentate Gyrus Using Thymidine Analogous -- Electroporation Techniques to Target Subependymal Zone Neurogenic Niche -- In Vitro and In Vivo Direct Reprogramming of Astrocytes to Induced-Neurons -- Retrovirus-Mediated Reprogramming of Endogenous Hippocampal Glia into GABAergic Induced Neurons -- Gene Expression Manipulation via Acute Electroporation in Human Brain Organoids -- Targeted Human Editing Using CRISPR Technology in Neural Stem Cells -- Patch-Clamp Recordings in Isolated Neural Cells, Acute Tissue Slices, and Brain Rosettes -- Acute Slice Patch-Clamp Recordings to Characterize Neural Populations in the Hippocampal Subgranular Zone -- Intracellular Calcium Recording and Immunostaining of Single Neural Populations In Vitro -- Cell Population Analyses of Neurospheres Generated In Vitro from Ovarian Cortical Cells in Mammals: Characterization by Immunohistochemistry and Flow Cytometry -- Reference-Based Gene Expression Analysis Using Galaxy Server -- Development of Novel Neuroprotective Agents Targeting GPCR Using In Vitro Assays -- Picrotoxin-Induced Epileptic Hippocampal Organotypic Slice Cultures (hOTCs). |